FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13829543
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG148820.10761020808858254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG14200.050.7771228
TAGCATC14400.040.34670636
CACGTCT18950.039.1548914
ACGTCTG19750.037.39151815
ACACGTC20350.036.63383513
CTGAACT23100.031.96882419
GGTAGCA22600.031.1288234
TCAGATT25100.030.5395552
TATCTCG25200.030.0025
GAACTCC24400.029.83546621
TTGAACG26950.028.7018667
GGATATC28650.026.755422
GTCACGG27800.026.69194629
CAGATTG29650.026.20723
GAGCACA29250.025.966239
ATATCTC29750.025.6484934
GATATCT31650.024.3299543
AGATTGA33650.023.7160364
TATGCCG5750.023.13030646
GTTTTAC30400.023.0282691
Produced by FastQC (version 0.11.2)