FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16686660
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACCTGGTGTG217720.13047548161225794No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG212760.12750304734440565No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT189000.11326412835162937No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACC183860.11018382348534697No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA179970.10785261999705154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG23600.057.39510328
CTCGTAT20700.056.30418444
TATGCCG21000.055.66650448
TCGTATG22000.055.20438845
GTCACGA25950.052.1971629
GTATGCC23050.051.7785947
CACGAGT26650.051.08833731
ATGCCGT25100.046.0153949
CACGTCT30800.045.22727614
TGGATAT29900.044.59837
GCCGTCT23400.044.5721251
ACGTCTG31600.044.1930415
ACACGTC32800.042.78976413
CTGAACT33250.042.1045119
TGCCGTC26550.041.78857850
GAACTCC34250.040.67092521
CGTATGC30400.039.37488646
TCACGAG35900.038.9001930
GTGGATA36250.037.0756436
GAGCACA39750.035.2206549