FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16833913
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG204260.12133839589167415No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT191150.11355054525944146No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACCTGGTGTG188250.111827832304943No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA176760.10500232477143015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA19350.057.3382136
CGTACGT21700.051.45165634
CCGTACG21850.051.09828633
ACGTAAT22750.049.07637837
GTATGCC11250.047.28850647
TCGTATG10850.045.48350545
CTCGTAT10950.043.46996744
CACGTCT25850.043.19152514
TATGCCG12000.043.1663248
ACGTCTG26450.042.0789315
ACACGTC26750.041.60763513
CTGAACT27350.041.5902819
GAACTCC29800.037.818621
ATGCCGT12400.037.5400249
GTCACCG30100.037.09360529
TAATCTC17100.036.0229940
TCAGATT21500.035.163972
GAGCACA32000.035.0004439
GGATATC37150.033.3523982
TATCTCG36150.033.3068855
Produced by FastQC (version 0.11.2)