FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20629608
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG231580.11225613206028928No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACCTGGTGTG229060.11103458679389351No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT224210.10868359689626676No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA219810.10655074008192499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT18700.052.0338129
CACGTTT18900.051.48206731
AGTCACG18850.051.43419328
ACGTTTC20600.047.23354332
CGTTTCG20900.046.38797433
TTCGGAA21900.043.94920336
ACGTCTG24450.040.94056315
CACGTCT24900.040.4822914
ACACGTC26250.038.53423713
CTGAACT27300.036.2817619
TCAGATT25500.033.903412
GAACTCC29200.033.68115621
TATCTCG44550.033.547935
GGATATC47500.031.6855682
ATATCTC47700.031.6260194
GAGCACA33700.030.4308349
CAGATTG29250.029.9158123
GATATCT52750.028.9965063
CTTGTCT49650.028.2694281
GGCAACG20200.027.8972362
Produced by FastQC (version 0.11.2)