FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14645110
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG166280.11353960468716177No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAAC154120.10523649190753773No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT150130.10251203302672358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT30150.055.37369514
ACGTCTG31750.052.91338315
ACACGTC32200.051.84923613
GAATATC30750.049.51185236
CTGAACT35250.047.75853319
AATATCG24350.045.42047537
GAACTCC37500.044.42666221
GAGCACA37850.044.3870939
GTCACCG37200.043.8442329
ATATCGG18450.042.30308538
TCAGATT28400.036.726352
AGTCACC55900.034.0610528
CAGATTG30550.033.7979743
TTGAACG30100.033.7217337
TATCTCG24850.032.113445
AGATTGA34450.031.2926884
CAGTCAC62000.031.04838627
GGATATC26600.030.790312
GTCTGAA57700.029.7833617
CTTGTCT44450.029.1352481
Produced by FastQC (version 0.11.2)