FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14852730
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG165760.11160237882194048No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG154230.10383949617343075No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAAC153770.10352978879976946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG16400.058.90172243
CTCGTAT15800.056.26513742
GTATGCC17200.054.53421845
TATGCCG17850.052.54837846
CACGTCT23500.051.68074414
ACGTCTG24350.049.58888215
ACACGTC25050.048.4834213
CTGAACT26050.047.15873319
ATGCCGT19300.046.424347
TCAGATT30600.045.9824642
CACCCAA27400.044.7089931
GCCGTCT16500.043.90840549
GAACTCC28400.043.25636721
GAGCACA29550.041.9301159
CAGATTG33950.041.5482643
TTGAACG33450.041.5407837
TGCCGTC19850.040.5536648
CGTATGC23550.039.97828344
AGATTGA36700.038.721094
TATCTCG17500.031.801225
Produced by FastQC (version 0.11.2)