FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12288575
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG130100.10587069696852563No Hit
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT128420.10450357344118419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT29300.054.11314814
ACGTCTG29300.053.75434515
ACACGTC30200.052.38588713
CTGAACT31450.049.74489219
CATTTTA32750.046.1680134
GAGCACA34650.045.85949
ATTTTAT32600.045.52079835
GAACTCC35250.044.28303521
TCAGATT24000.040.689052
TTGAACG26200.037.9396067
TTATCGC9800.037.4991438
CAGATTG27100.037.0678333
AGTCACC50600.035.2087128
AGATTGA29000.035.0013354
CAGTCAC55500.032.73084627
TGTAAAA11400.032.23610362
TTATCGG12250.030.2850238
CACCATT52300.029.71335831
TGTTTAC34400.029.6084465
ACCATTT52250.029.54083432
Produced by FastQC (version 0.11.2)