FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10931969
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG127610.11673102988125927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT29350.057.47870314
ACGTCTG29950.056.32668715
ACACGTC30050.056.02329313
CTGAACT32300.051.57823619
GAGCACA33650.050.3429039
GAACTCC33900.048.62764721
CATGGCA33100.047.90095534
GTCTGAA47500.036.252317
AGTCACC50300.035.76655228
CAGTCAC53600.034.28249727
TCAGATT18500.033.298212
TTGAACG19150.031.4367477
CAGATTG19500.031.411263
AGCACAC54850.031.14003810
AACTCCA59300.029.4516722
TCTGAAC57850.029.34299318
TATCTCG13400.028.471455
AGATTGA22250.027.8437144
GCACACG62550.027.41831611
CCATGGC62950.026.3546133
Produced by FastQC (version 0.11.2)