FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11198895
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG175880.15705120907018058No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA143050.1277358167926389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGATT31950.046.8878022
ACGTCTG15900.046.6666215
CACGTCT16100.046.3045114
ACACGTC16200.046.01908513
TTGAACG33700.044.1406027
CAGATTG34550.043.258043
AGATTGA36500.041.905944
CTGAACT17850.040.97998419
GAACTCC19000.038.49944321
GAGCACA19900.037.8148779
GATTGAA44550.034.0980075
CTTGTCT18600.030.4864251
AACGCTG51150.030.10816810
CAGGCCT50700.029.33882921
GTATGCC5500.029.27256645
TGTTTAC23050.029.1552855
TATGCCG5100.028.13709846
CTCAGAT60000.027.1856121
CCTAACA55450.027.07866525
TATCTCG11650.026.7393655
Produced by FastQC (version 0.11.2)