FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13079460
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG215460.16473157148689627No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA158400.12110591721676583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCAAT25350.055.22620435
TCGTATG10850.054.51572443
CACGTCT28200.052.74803514
CCACTCA28000.052.25000833
CTCGTAT11000.052.18142742
ACGTCTG28600.052.1326815
GTATGCC12550.051.87192545
CTCAATC23200.050.23650436
ACACGTC30000.049.69982513
CTGAACT30400.049.6213419
GAACTCC30700.048.68023321
TATGCCG12850.048.2095746
TCAGATT33450.046.563652
CACCACT31950.046.55730431
GAGCACA33100.045.468469
TTGAACG36600.042.5548177
CAGATTG37750.041.9087373
AGATTGA38550.041.3114174
TCAATCT22300.040.49266437
ATGCCGT13200.040.3027347
Produced by FastQC (version 0.11.2)