FastQCFastQC Report
Wed 1 Aug 2018
HWCMHBGX5_n01_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCMHBGX5_n01_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18549052
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTAT270550.14585651061844024No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG203220.10955815962993688No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG193390.10425869742561507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAT28550.056.0243735
TATGCCG16750.051.8204846
CTCGTAT17900.050.44651442
TCGTATG17350.049.01977543
GTATGCC18350.048.44647645
CGATATC31200.048.3492336
CACGTCT35100.046.66679414
ACGTCTG35600.045.81448715
ACACGTC37150.044.46885313
ACCACGA36700.044.44177632
CTGAACT38700.042.32489819
CCACGAT39050.041.9464533
TCAGATT34900.041.620372
ATGCCGT19850.041.25933547
GAACTCC40450.040.58041821
TTGAACG35650.040.350857
CAGATTG36450.039.562433
GAGCACA44100.037.698929
AGATTGA39050.037.5556344
CTTGTCT36300.037.4120831
Produced by FastQC (version 0.11.2)