FastQCFastQC Report
Sat 15 Apr 2023
HWCM5BGXN_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCM5BGXN_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293611124
Sequences flagged as poor quality0
Sequence length16
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCGCCGTATCAT257429838.767713787301872Illumina Single End PCR Primer 1 (100% over 16bp)
ATGTTTCACAACAACC11565420.39390265063662916No Hit
ACGGAGTCTTAGCCAC8012520.2728956550024991No Hit
CTGAAGGTGGAGGAAG7907980.2693351632004242No Hit
TGTCAGGACGTGCAAT6452460.21976210955821962No Hit
GTTGAGCGATGGCGCA6180690.21050598886709757No Hit
TCCGGTTGACATAGAC5293260.18028131658935376No Hit
ACCTTGCACCTCAGGA5044880.17182182784055552No Hit
GCCAGCTCTTGACCGT4533180.1543940140360622No Hit
TGACAACGAAACCGGT4470770.1522684133725124No Hit
TGCTATGTGCTTGTTT4456710.15178954868208602No Hit
TTACGTCACATTGACG4294090.1462509301929582No Hit
CAAGTCCCTCCCTCAC4163260.14179503634882717No Hit
CCTTTATTGAGCCTTG3699150.12598807393959638No Hit
TAACGTGGAGCTTATC3648410.12425993778083148No Hit
GCATAATGATAACGGT3592460.12235435602909923No Hit
TCCGCCAACTTGTTGC3559700.12123859448867474No Hit
GCCAAATACAATACCG3508720.11950228425269066No Hit
ATTAAGGCTTAGCCAC3446040.11736748775226923No Hit
GTGTTTGCTATCCTCC3424710.11664101663941043No Hit
GCCAACAGATCAAGAC3419330.11645778107507944No Hit
TAGGTTGGACATTGAG3354000.11423272913869571No Hit
TCCACAATGACTAAGG3329980.11341464024367143No Hit
GACCAGGTGACTTACA3239420.11033028843961648No Hit
TCCATAGTGGCTTGAC3223280.10978058174662347No Hit
GCAACTAACAAGCTAC3207390.10923938971740049No Hit
CTTCATTTGATGCACC3204250.10913244554044893No Hit
TGTCAATCTTGCATGC3154470.10743700569056096No Hit
TGCGCCTACACAAGCT3147730.107207450355321No Hit
ATGGTTAGAACAAGTT3077410.10481244573008752No Hit
TACGGACACCCTTCTC3071440.10460911555925927No Hit
CGAGCTGGACACTAAG3054990.10404885068319142No Hit
ATGAGAAACACCATGA3015370.10269944676891739No Hit
AACGCTAACCCAACCC2954320.10062016587627655No Hit
CGGGAACCTATTGACC2945740.10032794261568918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)