FastQCFastQC Report
Sat 15 Apr 2023
HWCM5BGXN_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCM5BGXN_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293611124
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC9732385333.147195403945254Illumina Single End PCR Primer 1 (100% over 8bp)
CTATTTGG7475203825.459538787774267No Hit
AACGCGAA6538583022.26953431096841No Hit
GGGGGGGG2993979110.19709014839642No Hit
GCGCACCT91839393.1279261067778887No Hit
CTATTGGA10142910.345453873198619No Hit
CTATTTGA10112640.344422917709344No Hit
GCGCGCCT5340710.18189739977290506No Hit
TATTTGGA3954440.13468290799499816No Hit
GCGCACCG3710100.1263610162127236No Hit
TGTAGATA3686190.12554667376975812No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)