FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27824405
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288899010.382935412275662No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACA353120.12691017112495306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG204650.036.516372
CGCAGAG265500.028.89176216
ATCAACG256200.028.85208111
ACATGGG253150.028.84675824
TACATGG251150.028.69761823
AGAGTAC260350.028.65959719
GTACATG254650.028.63062922
CAGAGTA263750.028.56759818
GAGTACA263300.028.25835820
CATGGGG170300.028.116325
AGTACAT261600.028.11485521
AAGCAGT277650.027.411591
CATGGGA72250.027.30103925
GTATCAA278750.026.1487469
ATGGGGA46950.025.15536326
GCAGTGG310900.024.8170153
GGTATCA305800.024.2239068
CAACGCA309200.024.1978413
CAGTGGT316600.023.8063374
GCAGAGT320700.023.69229317
Produced by FastQC (version 0.11.5)