FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n02_190222-lib2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n02_190222-lib2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59217683
Sequences flagged as poor quality0
Sequence length49
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACA5141380.8682170155154499No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGAATTCCGC888690.1500717277303808No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG864880.1460509692687571No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT804540.13586144530511268No Hit
GTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGACCTCGAT684840.11564788848628205No Hit
ATATAATTTTGGGTGTAGCCGTTCAAATTTTAAGTCTGTTCGACTTTTA620640.10480653219748566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2423700.039.7200781
AGCAGTG2527100.037.9832272
GCAGTGG2836350.033.8213423
CAGTGGT2896600.033.0578354
AGTGGTA2992450.032.0457735
GTGGTAT3105350.030.8314256
TGGTATC3102250.030.815337
GGTATCA3153550.030.3322728
TCAACGC3215250.029.58586112
CAACGCA3224350.029.54324713
ATCAACG3250100.029.32793611
CGCAGAG3262300.029.31239116
GCAGAGT3258450.029.25829517
AACGCAG3262400.029.20855914
ACGCAGA3268850.029.12053915
AGAGTAC3273900.029.09825119
TACATGG3251100.028.97726423
ACATGGG3240750.028.95318224
CAGAGTA3304150.028.91065818
GTACATG3277950.028.85133622
Produced by FastQC (version 0.11.5)