FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n02_190222-lib2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n02_190222-lib2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55651406
Sequences flagged as poor quality0
Sequence length49
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACA4781140.859122948304307No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGAATTCCGC848110.1523968684636647No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG817530.1469019488923604No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT749740.13472076518605838No Hit
GTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGACCTCGAT680930.12235629770072655No Hit
ATATAATTTTGGGTGTAGCCGTTCAAATTTTAAGTCTGTTCGACTTTTA584240.10498207358858103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2247700.039.7022971
AGCAGTG2339250.038.044632
GCAGTGG2621700.033.9015463
CAGTGGT2690650.032.9786264
AGTGGTA2777250.031.9705925
GTGGTAT2889300.030.6906016
TGGTATC2888600.030.6525217
GGTATCA2933050.030.2177438
TCAACGC2994050.029.44480512
CAACGCA2998650.029.41571413
ATCAACG3017600.029.28233311
CGCAGAG3035950.029.1451416
GCAGAGT3030750.029.11241117
AACGCAG3033600.029.10184714
ACGCAGA3040600.028.9930315
AGAGTAC3046750.028.94974119
TACATGG3021450.028.86358623
ACATGGG3012800.028.80331824
CAGAGTA3075950.028.7458118
GTACATG3048650.028.721222
Produced by FastQC (version 0.11.5)