FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n02_190222-lib2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n02_190222-lib2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58340353
Sequences flagged as poor quality0
Sequence length49
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACA5051650.8658929437742688No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGAATTCCGC906090.15531102460075963No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT795500.1363550199979078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG756790.12971981845910327No Hit
GTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGACCTCGAT694590.11905824429961882No Hit
ATATAATTTTGGGTGTAGCCGTTCAAATTTTAAGTCTGTTCGACTTTTA624830.10710082607830639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2354650.039.7631721
AGCAGTG2457550.037.967932
GCAGTGG2757650.033.8249473
CAGTGGT2825900.032.936664
AGTGGTA2921300.031.9277445
GTGGTAT3037400.030.675616
TGGTATC3036600.030.6251547
GGTATCA3085900.030.1621678
TCAACGC3146800.029.40098812
CAACGCA3157850.029.33805713
ATCAACG3181150.029.14780211
CGCAGAG3195900.029.0916516
GCAGAGT3187750.029.06024417
AACGCAG3192550.029.03568514
AGAGTAC3204150.028.9374219
ACGCAGA3202350.028.92196515
TACATGG3181600.028.7954623
ACATGGG3173100.028.75687824
CAGAGTA3232550.028.74231518
GTACATG3207600.028.67656722
Produced by FastQC (version 0.11.5)