FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n02_190222-lib2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n02_190222-lib2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53648304
Sequences flagged as poor quality0
Sequence length49
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACA4608750.8590672316500443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGAATTCCGC835860.1558036205580702No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT725170.13517109506388122No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG719090.1340377880352005No Hit
GTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGACCTCGAT663130.12360688979096153No Hit
ATATAATTTTGGGTGTAGCCGTTCAAATTTTAAGTCTGTTCGACTTTTA564540.10522979440319305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2160100.039.664061
AGCAGTG2254250.037.9055562
GCAGTGG2527600.033.7763863
CAGTGGT2587000.032.937694
AGTGGTA2674050.031.9136685
GTGGTAT2778500.030.6685036
TGGTATC2777250.030.6117827
GGTATCA2819450.030.1570368
TCAACGC2871600.029.44389312
CAACGCA2884250.029.35108813
CGCAGAG2918200.029.12764216
ATCAACG2912000.029.10753611
GCAGAGT2914500.029.07877317
AACGCAG2918700.029.02427914
ACGCAGA2924850.028.94897515
AGAGTAC2930350.028.92141719
TACATGG2909100.028.79307623
ACATGGG2897500.028.76363624
CAGAGTA2955850.028.74664918
GTACATG2933950.028.64512422
Produced by FastQC (version 0.11.5)