FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n02_190222-lib1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n02_190222-lib1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28671133
Sequences flagged as poor quality0
Sequence length49
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACA2202640.7682430966366066No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG893040.3114770525461969No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT409190.1427184618061658No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGAATTCCGC330200.11516810305333941No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG310130.108168030890164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT873000.039.1993641
AGCAGTG946650.036.0909582
GCAGTGG1086600.031.4563563
CAGTGGT1093700.031.1379854
AGTGGTA1107500.030.7784355
TGGTATC1142600.029.7148727
GTGGTAT1147750.029.6514196
GGTATCA1161900.029.2429878
TCAACGC1190350.028.2699112
CAACGCA1196750.028.13865513
GCAGAGT1211450.027.89347317
ATCAACG1209750.027.89186511
CGCAGAG1219350.027.82086216
AGAGTAC1215000.027.7866219
AACGCAG1217500.027.71197314
ACGCAGA1221100.027.61949315
CAGAGTA1230150.027.53152318
TACATGG1218600.027.3901823
GTACATG1227250.027.3351722
ACATGGG1217100.027.324724
Produced by FastQC (version 0.11.5)