FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n02_190222-lib1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n02_190222-lib1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27551145
Sequences flagged as poor quality0
Sequence length49
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACA2124370.7710641427062287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG846350.3071923145117925No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT396330.14385246057831716No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGAATTCCGC327160.11874642596523666No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG297230.10788299361061038No Hit
GCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGACCTCG277210.10061650795275479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT842550.039.159421
AGCAGTG911100.036.0964282
GCAGTGG1044850.031.4818213
CAGTGGT1048400.031.2927114
AGTGGTA1058400.031.0512165
GTGGTAT1092800.030.0496356
TGGTATC1090850.030.0313747
GGTATCA1111700.029.4669468
TCAACGC1141000.028.54349512
CAACGCA1146400.028.39829313
GCAGAGT1156450.028.19032117
ATCAACG1158850.028.16964311
CGCAGAG1161200.028.15509416
AACGCAG1159450.028.09687614
AGAGTAC1162200.028.03931219
ACGCAGA1163750.027.99648715
CAGAGTA1176950.027.75515718
TACATGG1166000.027.6571923
ACATGGG1163150.027.58404524
GTACATG1174500.027.5196222
Produced by FastQC (version 0.11.5)