FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n02_190222-lib1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n02_190222-lib1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44798115
Sequences flagged as poor quality0
Sequence length49
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACA3566870.796209840525656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1298370.2898269268695792No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT670370.14964245705427562No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGAATTCCGC532190.11879740922134782No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG503770.11245339229117117No Hit
GCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGACCTCG455970.10178330048038853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1389900.038.9672851
AGCAGTG1505650.035.8679282
GCAGTGG1731400.031.1798863
CAGTGGT1739350.030.9586814
AGTGGTA1765600.030.5222875
TGGTATC1819950.029.5526527
GTGGTAT1824650.029.530266
GGTATCA1855600.029.032398
TCAACGC1901650.028.14074512
CAACGCA1911900.028.03160313
AACGCAG1936700.027.72361614
ATCAACG1935250.027.71847711
GCAGAGT1934800.027.70401217
CGCAGAG1944500.027.68708416
AGAGTAC1936850.027.67793819
ACGCAGA1942800.027.58798215
CAGAGTA1964100.027.34535418
TACATGG1941850.027.26357823
ACATGGG1940850.027.17300824
GTACATG1958500.027.13152722