FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n02_190222-lib1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n02_190222-lib1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27842550
Sequences flagged as poor quality0
Sequence length49
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACA2143210.7697606720648792No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG858940.3084990419340182No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT399430.14346027932068003No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGAATTCCGC329700.11841587785601534No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG298220.10710944220267181No Hit
GCGTAATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGACCTCG279190.10027457973497399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT842150.039.0887221
AGCAGTG922000.035.6208762
GCAGTGG1055900.031.1118283
CAGTGGT1062150.030.8797284
AGTGGTA1076300.030.5192685
TGGTATC1111250.029.5021937
GTGGTAT1114100.029.4234966
GGTATCA1132750.028.9745988
TCAACGC1162850.027.97686412
CAACGCA1168500.027.88410813
ATCAACG1179850.027.6606311
AGAGTAC1182550.027.61206419
GCAGAGT1181650.027.5966217
CGCAGAG1184450.027.58606316
AACGCAG1182200.027.56860714
ACGCAGA1183550.027.48346715
CAGAGTA1196500.027.29538218
TACATGG1186650.027.21905723
CATGGGG792350.027.17029625
ACATGGG1184250.027.15946224
Produced by FastQC (version 0.11.5)