FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27824405
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG295233410.610591673029486No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGG429460.15434651702345478No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGG415040.14916401626557693No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGG377600.1357082029247346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT16700.017.7880551
AGGGGGG44650.017.4951631
TGGGCGT15050.017.2128071
CCGGGAT14900.017.1847081
CGGGGGG52400.017.0645451
ATGGGAG15850.016.9750651
GCGAAGA6800.016.9106794
TGCGTGG17600.016.8216081
TACGGGC16650.016.8202421
GGGACCT12450.016.710231
CGTGAGC16050.016.701221
GACTGCG18150.016.6425061
GACTAAC17000.016.5916141
GGGAATG16000.016.5658721
CGGCTAG21300.016.435271
AGGACGA6200.016.2881368
ACGAGGA17100.016.2606221
GGCTCGA16400.016.2228161
CCGGTAG20000.016.2032991
CCTACCA19100.016.0242061
Produced by FastQC (version 0.11.5)