FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n01_190222-lib2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n01_190222-lib2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59217683
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA225950.018.144648
GCGCGAT160900.017.8668041
CCGTGGA250000.017.7727551
CTGCGGA146600.017.692321
TGGGCGT157500.017.6428151
TTGAACG169000.017.6082611
GAATGAA183050.017.5518151
ACTGAAC161750.017.3895031
GACTAAC172850.017.2218481
CGGACTG146950.017.1668931
CATGGCG154300.017.1141051
ATAACGC162800.016.9947191
CGAGCAC152850.016.962341
CTCGAAA169050.016.9462641
GGCTCGA143200.016.8480821
CGTGAGC159000.016.8472751
CCTACCA212050.016.8165721
TCTGGAA166300.016.7633531
TGCGTGG163650.016.7536411
TACGGGC155750.016.6593531
Produced by FastQC (version 0.11.5)