FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n01_190222-lib2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n01_190222-lib2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55651406
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA211550.018.164818
GCGCGAT154250.017.9240511
CCGTGGA229350.017.9122141
TGGGCGT144500.017.8112831
CTGCGGA141800.017.7483391
GACTAAC158700.017.3710331
TTGAACG158800.017.3663921
ACTGAAC148700.017.3552971
CGGACTG136850.017.2354371
GAATGAA171300.017.214461
CGAGCAC149650.017.0847031
TGCGTGG155550.017.0733151
CATGGCG141000.016.9835431
CCTACCA196600.016.9020521
TGAGCCG158500.016.8312761
ACGAGGA148050.016.8099061
CGTGAGC148650.016.8093471
CCGGGAT117000.016.8081911
TACGGGC147150.016.8039571
GGCTCGA132000.016.8002241