FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n01_190222-lib2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n01_190222-lib2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58340353
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA222700.018.1174748
CTGCGGA142500.017.9978711
GCGCGAT158650.017.9059051
CCGTGGA241400.017.784451
TGGGCGT153450.017.593571
ACTGAAC156300.017.439161
CGGACTG142100.017.4290851
TTGAACG169150.017.3798331
GAATGAA185500.017.3146781
GACTAAC166000.017.2998811
CGAGCAC157100.017.1975421
TGCGTGG157000.016.9791361
CGTGAGC157650.016.9725781
GGGCATC147750.016.9656931
CATGGCG155100.016.9549621
CCTACCA206150.016.841831
ACGAGGA158200.016.8250521
CTCGAAA156400.016.8140321
ATAACGC152100.016.8027231
GGAAAGC122000.016.775051
Produced by FastQC (version 0.11.5)