FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n01_190222-lib2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n01_190222-lib2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53648304
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA207300.018.1513488
CCGTGGA216400.018.073121
CTGCGGA134300.017.8527721
GCGCGAT145750.017.836571
TTGAACG157550.017.5799711
ACTGAAC144900.017.471791
CGGACTG131700.017.4305291
TGGGCGT142100.017.3585191
GACTAAC153400.017.2796171
GAATGAA167200.017.2174241
CGAGCAC141600.017.1584431
ACGAGGA141250.017.009761
CCTACCA191850.016.9603921
GGCTCGA127250.016.9002951
CTCGAAA148500.016.8933091
ATAACGC147850.016.879631
GGGCATC135550.016.8321421
CGTGAGC152550.016.8251171
TGCGTGG145500.016.7466491
CATGGCG136250.016.723641
Produced by FastQC (version 0.11.5)