FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n01_190222-lib1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n01_190222-lib1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28671133
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC78800.017.9495451
GGGACCT71650.017.7303681
TGAGCCG81300.017.5821481
ACTGAAC77100.017.5539021
GCGCGAT76650.017.5134071
CTGCGGA55550.017.4129641
ATGGGAG90500.017.2206341
CGGACTG58750.017.1455671
ATAGACC61950.017.0834121
CAATGGA70750.017.0732528
TCTGGAA94900.017.0651821
ACGAGGA98300.017.0549621
AGCTTGA81000.017.0297991
CGCTGGA70350.017.020031
CACCGAA54200.017.0101188
TGCGAGG54300.016.9243454
ATGCGAT67400.016.904181
GAATGAA68550.016.8978461
GGCGACT75550.016.8548221
TACGGGC88450.016.7941821