FastQCFastQC Report
Wed 27 Feb 2019
HWCLJBGX9_n01_190222-lib1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCLJBGX9_n01_190222-lib1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27842550
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT72600.017.8968831
TGACGGC73100.017.788151
TGAGCCG78300.017.7309721
GGGACCT68750.017.720641
ACTGAAC74900.017.6544631
CAATGGA72950.017.5453328
ATGGGAG86850.017.3674621
CTGCGGA58400.017.3158231
GGCGACT71650.017.1988471
TCTGGAA92650.017.1655031
TGCGAGG50350.017.1198644
ATGCGAT64700.017.0365721
GAATGAA67150.016.9959131
ACGAGGA94650.016.9718721
GACTGCG88150.016.9184321
GATGAAA64350.016.8960761
CATGGCG75800.016.864231
TGCTACC78750.016.8421611
CGCTGGA68750.016.8331551
TACGGAT71550.016.80351
Produced by FastQC (version 0.11.5)