FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3427151
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG7100.022.8345188
GCCAGTA7400.021.914871
GTGCGAG8100.020.8671610
TGCGAGT9200.019.1220611
CTGACAT9850.017.865151
CGCGTAT4650.017.808651
TGTGTGC9950.016.2939787
CCCTGTG13000.015.92736863
GTACGGC3703.6379788E-1215.84891317
GTCGCTA12600.015.05742827
CGCTATG12450.014.96199129
TGTGCGA11300.014.6526149
CTGCCAG4251.8189894E-1214.6107982
CACGCTT11650.014.2163491
GCCGGAT4403.6379788E-1214.115381
GGCTGCC14100.013.94649643
GTCCTAT7700.013.88929943
ATGTGTG12550.013.744116
TTCGTAC11850.013.68144120
GAGCGAT10100.013.66126518
Produced by FastQC (version 0.11.2)