FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79781609
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG126600.018.826298
GTGCGAG146600.016.23437310
CGCGTAT87000.015.6653311
GGCGGGC185200.015.60714313
CGGCAAT236650.015.2943381
CGGGCGC201800.014.39167515
CGCTATG203950.013.96963829
TGCGAGT171450.013.92159711
TAGCCCG203650.013.85627334
GGCGCAT219500.013.48258617
GTCGCTA209750.013.43537427
GGCAATC276250.013.0874012
ATCGGGC244050.013.0609196
CTGACAT219850.012.9319021
GCCAGTA174050.012.8061971
TACGTGG192800.012.69042255
GCAATCG280200.012.5702033
CTACGTG197050.012.31159654
CGTTCAG222800.012.14366257
TGTGCGA200300.012.1058369
Produced by FastQC (version 0.11.2)