FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21070084
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG26300.016.1311118
GGCGGGC30500.015.83226713
CGCGTAT17500.015.1811141
CGGCAAT51200.014.4883681
CGGGCGC33500.014.41445215
GTGCGAG32900.012.89508210
TAGCCCG38650.012.58471134
ATCGGGC45450.011.9922586
GCGGGCG40700.011.94922214
GGGGCGA23800.011.89101264
GGCGCAT41100.011.83292817
GGCAATC65950.011.5080062
GCAATCG65050.011.2957283
CGCGAAT28350.011.19658851
TCGGGCG46850.011.1904847
TGCGAGT38450.011.12346911
ATAGGGG27000.010.86466761
CCGACAT73050.010.7687571
TGTGCGA42250.010.4495649
CGCGCGT22900.010.39911865
Produced by FastQC (version 0.11.2)