FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52854446
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT157500.015.028021
GTGTGCG85650.014.9003958
GGCGGGC134300.014.20275413
TACGTGG133000.013.67382355
CTACGTG136750.013.29885554
GGCAATC182250.013.2693962
CGGGCGC145950.013.25812915
TAGCCCG142150.013.25026934
CGCTATG128550.013.120934529
GTCGCTA130700.013.03707427
CGCGTAT53750.012.9667491
CGCGAAT77850.012.9414351
GCTACGT143300.012.85901153
ATCGGGC164000.012.7891696
GGCGCAT154050.012.67296317
CGCGGGG43250.012.5248271
GTGCGAG102400.012.39570710
GCAATCG192900.012.3398813
GCGATTT190400.012.2138111
GCCAGTA134100.012.1700011
Produced by FastQC (version 0.11.2)