FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3589691
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG5550.021.1303488
CGCGTAT4300.017.6510981
GTGCGAG7000.015.27511110
CGCTATG9050.015.24558929
GTCGCTA8850.015.19994527
CTGACAT9500.014.5262621
CGGCAAT10750.013.799951
GGCGGGC8800.013.71848213
TGCGAGT8100.013.62654111
ACATGTG12900.013.10277754
ACATCCG8050.012.8606855
CGTACTG10250.012.78734722
GCCAGTA8100.012.777731
GCCGGAT5257.2759576E-1212.4856681
CGGGCGC9950.012.47958615
CCGACAT16200.012.3518061
CGTTCAG10400.012.27758657
ATCGGGC11550.012.2450046
CCCTGTG10450.012.21935363
GCGATTT12200.012.1597911
Produced by FastQC (version 0.11.2)