FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9875317
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG20250.022.1432518
GTGCGAG22950.019.8387510
GTCGCTA29000.018.9117227
CGCTATG29850.018.37310229
CGCGTAT11950.017.9024261
TGCGAGT25650.017.48151611
TGTGTGC27300.017.1830167
TGTGCGA27300.016.5512879
CTGACAT32700.015.3005941
CCGGCAT15400.015.0121241
CCGACAT48100.014.7778151
CCCTGTG33950.014.6388763
GGCTGCC39450.014.16739743
GACATCG42400.014.1570413
TAGCCCG24250.014.08270634
GGCGGGC23800.014.057816513
CCCGTAA31200.013.70975833
CGGCAAT30900.013.5118531
GCTATGA40850.013.510344530
GCAACGT41250.013.29551222
Produced by FastQC (version 0.11.2)