FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17868018
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA34100.026.6107331
TATCTAG36400.021.9874065
CCGGCAT14400.021.0850981
GGCAGAC20400.020.4649491
CTGACAT49550.019.7059881
GTGTGCG47800.019.3388188
AGGCGTT77100.019.29106354
CGGCAAT86600.019.2833711
GGCAATC87850.019.2028392
CGTTCAG80150.019.03095457
GCAATCG88200.019.0090833
CAGACGT22500.018.3989143
TGTGTGC50150.018.2950537
TGACTTA81350.018.19406346
AGAGGCG84500.018.13171452
ACATCCG36850.017.98030355
GTGCGAG51650.017.96407910
CATATCT46950.017.708223
ATGTGTG52900.017.6074126
GGCGGGC92700.017.56245613
Produced by FastQC (version 0.11.2)