FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17665735
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA30000.021.62171
CGCGTAT23300.017.7696381
GTGTGCG36000.017.4371648
CGGCAAT62300.017.389811
CGCGAAT34850.016.7315881
GCAATCG63450.016.038273
GGCAATC66800.015.9572862
CGCTATG53600.015.89435129
CCGGCAT16200.015.7604991
GTGCGAG39350.015.60207110
GGCAGAC23750.015.5443811
TACGTGG45050.015.397943555
GGCGGGC61000.015.15346313
GTCGCTA57650.015.13676527
CTACGTG46450.014.85951154
ACATCCG34900.014.83294255
CTGACAT47250.014.8233891
GTACGGC22600.014.80369917
TGTGTGC44350.014.6208027
GGCGCAT63700.014.56531217
Produced by FastQC (version 0.11.2)