FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224060
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG7800.018.13117429
GTGTGCG5650.017.7037818
CGCGTAT4050.016.1868861
GTCGCTA8600.016.04371627
TGCGCTA3705.4569682E-1114.9241967
CCCTGTG9750.014.513267563
CGGCAAT9750.014.5092141
TCCTATG12650.014.45920460
GTGCGAG6600.014.11030210
GGCGGGC6250.013.79674113
CCTAGGC5750.013.796748
GCCAGTA7950.013.4542421
GACTGTC12650.013.362764
TGCGAGT7100.013.11661811
CGCTAGT5003.6379788E-1213.11422459
CCTGTTA16350.013.07947811
TACTCCG13750.013.04479823
CACGAAT9050.012.96268751
ATCCCGA13450.012.82344230
ATCGGGC7900.012.663536
Produced by FastQC (version 0.11.2)