FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7107776
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG12350.022.0646118
GCCAGTA13900.019.612311
GTGCGAG14500.019.26873410
TGCGAGT15350.018.65116111
CTGACAT18300.018.1024191
GGCGGGC17850.017.39163613
CGCGTAT7800.017.2538661
CGGCAAT19850.016.5150361
GGCGCAT19800.015.85303217
TGTGTGC17700.015.7851227
CCGGCAT8100.015.7627931
TAGCCCG19250.015.41177834
GCAATCG23250.014.8393223
CGGGCGC20850.014.55837115
TGTGCGA19750.014.3213169
CGCGAAT14000.014.2960621
GGCAATC23750.014.09170152
CCTAGGC14100.013.9441458
CTGCGCC19300.013.9403439
CGTTCAG20900.013.86894257
Produced by FastQC (version 0.11.2)