FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7362172
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT6550.019.4905341
GTGTGCG9150.014.3244638
GGCGGGC10600.013.66656113
CGCTATG14600.013.2297329
CGGCAAT18100.012.7720191
ATCGGGC12200.012.7240266
GCGGGCG11700.012.3816714
CGCGCGG7000.012.32092536
TCGGGCG12400.012.2390247
CGGGCGC12100.011.9723615
CCGACAT25450.011.9304871
TAGCCCG12050.011.45119134
GCGCGGT8350.011.15530937
GTGCGAG11150.011.13636410
GGCAATC20200.010.9300992
GCGTATT12700.010.865552
CGTTCAG13750.010.79275657
GTCGCTA17300.010.76651827
AATCGGG18300.010.7447335
CCCTGTG18150.010.45845263
Produced by FastQC (version 0.11.2)