FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48272427
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA85600.019.8721331
GTGTGCG100400.018.8262338
CCGGCAT47150.017.5630871
GTGCGAG108900.017.32511110
CGGCAAT175400.016.7996271
CTGACAT128750.016.24041
TGCGAGT117650.016.1245411
CGCTATG149500.015.98878129
CGCGTAT62300.015.6736361
GCAATCG182300.015.6497983
TACGTGG136750.015.44414355
CTTCCGA105950.015.2736641
GGCGGGC172550.015.15202913
GGCAATC192850.015.0264942
CTACGTG141050.015.02225154
GTCGCTA161100.014.81614527
TGTGTGC131250.014.6639817
GCGATTT164900.014.6260781
GGCGCAT182350.014.50795417
AGGCGTT172000.014.265354
Produced by FastQC (version 0.11.2)