FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8124089
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA16900.021.6402551
GTGTGCG15700.019.5523938
GTGCGAG17200.017.8472410
CTGACAT21900.016.6995561
CGCGTAT9300.016.6944751
CGGCAAT29850.016.4129711
CCGGCAT8650.016.353511
GGCGGGC25800.015.90877613
TGCGAGT19600.015.83784111
GTCGCTA25050.015.42154327
CGCTATG24950.015.34529829
GCAATCG31750.014.5588393
GGCGCAT29800.014.35208417
GGCAATC31550.014.3232082
TAGCCCG27500.014.17496934
AGGCGTT26550.014.16837954
TTCGTAC28350.013.99126520
TGTGTGC22000.013.9532987
TACGTGG23750.013.8044255
CGGGCGC30250.013.79651815
Produced by FastQC (version 0.11.2)