FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4854782
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT15300.018.0407641
GTACGGC4450.017.05219817
GGCGGGC12150.016.74917413
GGCAATC16900.015.9219842
GTGTGCG8550.015.7331688
TAGCCCG13000.015.65613434
CCGGCAT5800.015.46684551
ATCGGGC15100.015.3064236
CGGGCGC12650.015.26916215
GGCGCAT14450.014.32191717
CGCTATG13150.014.16399329
GTGCGAG10400.013.92943910
TGCGAGT10750.013.79677911
GTCGCTA13800.013.7467927
GGGGCGA7600.013.62394264
TCGGGCG16300.013.3312437
CGCGGGG4204.129106E-1013.1439851
GCAATCG17500.013.0101333
CGTTCAG14750.012.86842857
CGCGTAT4752.2009772E-1012.3484291
Produced by FastQC (version 0.11.2)