FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6650912
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG20100.020.7640129
GTCGCTA21850.018.3118327
GTGTGCG14150.018.2817178
GCCAGTA17850.017.3976081
CCTACTA11600.016.9504534
CCCTGTG24350.016.29967163
GGCGGGC18150.015.9630113
CGCGTAT7700.015.6842041
CCGGCAT7400.015.3874771
GGCTGCC26500.015.36194543
GTGCGAG16450.015.3062610
GACTGTC31400.015.2719764
CCGACAT35450.014.9895881
TAGCCCG19800.014.8087434
AGGCGTT18900.014.60748654
CGGGCGC20150.014.54976715
GACATCG28850.014.469373
CGGCAAT23200.014.42676451
CGTTCAG20150.014.38670457
GCTATGA29150.014.31776530
Produced by FastQC (version 0.11.2)