FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308976
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT851.4423262E-424.3557341
CCTACTA2450.023.9378634
CGCGTAT1752.401066E-1023.6598571
TATCAGG3000.020.70553858
GGCCCGC2352.0008883E-1120.55633448
GACTATC2700.020.44991355
AATAGGG2303.45608E-1019.50000236
GGGGCGA3400.019.2845764
GTGCGAG2901.8189894E-1219.0301210
CCTGCCA2759.094947E-1218.8203371
GTGTGCG2951.8189894E-1218.7075778
GTACTCT3550.018.4626733
ATAGGGG3650.017.96233661
CGCTATG3650.017.95478829
CGCGGCC2701.5097612E-1017.88957245
CCCCGAT1954.5887464E-717.6943361
CTGGCGG1954.6034802E-717.6889326
GACTGTC8650.017.5471214
ATCTAAC3153.6379788E-1217.52447940
TCCTATG8800.017.25395660
Produced by FastQC (version 0.11.2)