FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4665845
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG4100.020.1907338
CGGGCGC4750.015.24931715
GGCGGGC4950.013.93636513
GTGCGAG5700.013.91802810
GGGGCGA4002.7921487E-912.94190164
CGGACAT5205.456968E-1212.6069481
CGCAAAT8550.012.5099421
CGGCAAT9700.012.0939141
GCGGGCG5501.6370905E-1111.91559314
CGCGAAT6109.094947E-1211.3125331
TGTGCGA7800.011.055299
CGCGTAT2954.3418657E-410.5264081
ATAGGGG4602.6820817E-710.50289561
ATCGGGC6909.094947E-1210.4989566
ACCTAGG7301.8189894E-1210.3950017
GGCGCAT6756.548362E-1110.22000117
ACTCACG10950.010.081196
ACCCGCT8900.010.077656
TACCTAG7605.456968E-129.9858496
TCGGGCG7001.3278623E-109.8550017
Produced by FastQC (version 0.11.2)