FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4140260
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCAG8600.022.464572
CCTGCCA8850.022.2219451
GTACTCT8400.022.1772293
TATCTAG8550.020.980935
CCTACTA8500.020.69774634
TGACTTA15400.020.61088446
AGGCGTT15100.020.5678854
CGTTCAG15800.020.31235557
ACATCCG9750.020.174155
TGGACAC9100.020.08927728
AGGACTG8800.020.00083562
CTAATGC9400.019.44789521
GACTATC7850.019.34224955
GGACTGT7750.019.1488763
CGCTATG18650.019.04970429
ATGCTGG9250.019.01748324
ACACCTA9050.018.67662231
CGGCAAT19300.018.413251
AGAGGCG17250.018.40377652
ATCTAAC9600.018.32834240
Produced by FastQC (version 0.11.2)