FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7426187
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG16500.018.6050578
CGCTATG19700.017.15917629
GTGCGAG18550.016.54897110
CCGGCAT9150.015.8379631
TGCGAGT19500.015.56585611
GTCGCTA22200.015.07136127
CTTCCGA21650.014.98100951
TGTGCGA22900.013.5560129
GGCGGGC20200.013.48964913
CGCGTAT9300.013.3564391
CGGCAAT26650.013.3355571
CGTTCAG20250.013.12168257
TAGCCCG21600.013.09623434
AGGCGTT21150.013.05235354
CTGACAT25700.013.0229621
GACTGTC39150.012.9531344
CGTACTG21650.012.90490922
TGTGTGC25300.012.6790697
TTCGTAC22550.012.54273520
CCGACAT45300.012.4915641
Produced by FastQC (version 0.11.2)