FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44696211
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGGC72400.014.76878813
GTGTGCG56200.014.1774278
CGCGTAT38700.014.0868741
CGGCAAT103050.013.9622861
TACCTAG128150.013.6211096
CGGGCGC84950.013.15537915
GTGCGAG65050.012.35464910
GGCGCAT92600.012.10581817
ATCGGGC98350.012.1011116
TAGGCAC138350.012.09161610
CGCGAAT71950.011.9888541
TAGCCCG88550.011.95992334
CCTAGGC152050.011.47851858
GTCGAAT158500.011.3852071
GGCAATC135600.011.372162
GCGGGCG94350.011.33291214
CCGGCAT55800.011.2539681
GGGGCGA45850.011.14032464
TCGGGCG108150.011.098767
GCAATCG129950.010.9638123
Produced by FastQC (version 0.11.2)