FastQCFastQC Report
Tue 31 Oct 2017
HWCGNBGX3_n01_nxlight_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWCGNBGX3_n01_nxlight_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7481804
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG10000.017.2456428
GTGCGAG11050.016.5433310
CGCGTAT7100.016.5222531
CGGCAAT18200.014.0284281
TGCGAGT13500.013.28553111
GGCGGGC13000.013.0005613
TGTGCGA13850.012.9497969
TAGGGCG9550.012.2796189
ATCGGGC17100.012.1042276
CCGGCAT9500.011.6218431
CGGGCGC14550.011.37856815
TACCTAG17000.011.3637336
TCGGGCG17750.011.2703917
TAGCCCG14700.011.26404134
CGGACAT8400.011.0900411
CCTAGGC17450.011.0688358
GGCGCAT16000.010.99409717
TTCGTAC17650.010.94348320
GGCAATC23400.010.9093952
CCGACAT29350.010.8150461
Produced by FastQC (version 0.11.2)